FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51652598
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC98050.020.6002254
ACCGCGT98950.020.297926
GACCGCG111250.018.1155385
CAGACCG116000.017.9751323
CCGCGTT122150.016.442847
GCACTAC88600.016.37603425
TTCGGCG62200.015.88026844
CCCGTAT30000.014.8487881
CGCGTTC135200.014.8060828
TCGGCGC66750.014.69461945
GTCCGTT37900.014.2468591
TCAGACC153600.014.0875652
CGTACGC72950.013.9982525
CCGTACG75750.013.451134
GATTTAT140150.013.417035
AACGCTA47500.013.30624623
ACGCTAC48700.013.11722224
CTCCGAT53800.012.5036261
ATAATAC125250.012.31954517
CACTACA121750.012.268929526
Produced by FastQC (version 0.11.5)