FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45000073
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG832470.18499303323352387No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG829540.18434192317865794No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA759430.16876194845283918No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG580840.12907534616666066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG231100.018.77924345
GGCATAG265750.016.34700235
ATAGGGG277750.015.84540638
GAGCTTA277050.015.77634644
GTCACTC364650.015.3183978
TGGCATA287400.015.19538134
GGTCACT371800.015.0446517
ACCCGGC87600.015.0136725
GGAGGTC393450.014.6303814
GGGAGGT417550.014.4923533
ACTCTCC386050.014.47024311
AGGTCAC404100.014.1278236
GAGGTCA414600.014.0715925
CATAGGG312900.014.0504737
GTTGTCG37550.013.87877526
TCACTCT413100.013.6996589
CACTCTC408100.013.69547610
GCATAGG324900.013.53825136
ATAGCTC91150.013.52333939
TCACCCG105200.013.1459843
Produced by FastQC (version 0.11.5)