FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43721689
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG116200.011.80871845
ACCGGTA39900.011.61377445
ATAGGGG122550.011.53017238
GGCATAG122300.011.40466635
CATAGGG130350.010.68331237
GTCACTC180850.010.6106128
ACCCGGC70150.010.3612245
TACCGGT46300.010.10529344
TGGCATA139700.010.04874934
GGTCACT192400.09.9959187
ACTCTCC197300.09.95617611
GAGCTTA141650.09.9249944
ATAGCTC64150.09.78621939
GGGAGGT224350.09.7611293
TAGGGGA199100.09.69668839
AGGTCAC211050.09.67746
GCATAGG145000.09.66600136
GGAGGTC216500.09.5732264
TCTATAG250850.09.4746493
GTTGTCG20200.09.35648826
Produced by FastQC (version 0.11.5)