FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19893408
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT832750.41860600255119684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC158000.027.4707874
GACCGCG157300.027.3927735
CAGACCG160500.027.1270083
ACCGCGT159600.027.0262136
TCAGACC176850.025.5987572
CCGCGTT169850.025.368637
CGCGTTC174050.024.7688268
GAACGAT188700.022.86861645
CTCAGAC213100.022.807991
GCGTTCT193600.022.3479219
ATACGGA205750.020.9524733
AATACGG207850.020.7843432
TACGGAG206150.020.67159834
AGAACGA213500.020.3492144
CAATACG219900.019.90140731
ACGGAGA218400.019.65616435
CCCAATA229800.019.47490129
AAGAACG223650.019.38545643
CGTTCTC232200.019.34022710
CACTCCC241750.018.75408224
Produced by FastQC (version 0.11.5)