FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50790545
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA542390.10678956093107486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG145100.011.659698545
ATAGGGG150250.011.350224538
GGCATAG153100.011.12291435
CATAGGG166900.010.09573637
GGGAGGT260600.09.8009163
TTCTATA319750.09.6980952
GGAGGTC246700.09.6227454
ACCGGTA35550.09.55595845
ACTCTCC237350.09.52861411
GAGCTTA182300.09.4528444
GCATAGG181100.09.39115936
TTTCTAT338150.09.2911181
ACCCGGC75000.09.210445
TACCGGT37650.09.20186644
TGGCATA185000.09.16888134
GTCACTC251750.09.0712988
TAGGGGA247000.08.98168839
GGTCACT256750.08.9378587
CGTAGGG61250.08.92509237
ATAGCTC73150.08.82765939
Produced by FastQC (version 0.11.5)