FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14083320
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG340870.24203809897098125No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA323040.2293777319552492No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA301230.2138913267610194No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG281120.19961202330132383No Hit
CCTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA166330.11810425382651249No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG156910.11141549009750541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGC31200.020.986086
CAATATA34250.019.0494564
AATATAG35650.018.9964355
TATAGCT35100.018.7825687
TACAATA38500.017.6467652
GCTAAGT37300.017.00925814
ACAATAT40300.016.9146963
GGAGGTC143500.016.2268264
GGGAGGT147500.016.1521593
GTTGTCG39250.015.87840326
GAGGTCA152100.015.5910935
ATAGCTG44350.015.1696358
TAGCTGC44800.015.1679929
TGTCGTT42550.015.12127628
AGGTCAC157100.014.9669416
CTAAGTG42750.014.84182815
GTCGTTC43450.014.70388229
TGGGAGG132200.014.3284612
TTGTCGT46400.013.72251327
TAGCGAT2306.692062E-913.695785523
Produced by FastQC (version 0.11.5)