FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47508264
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA4231750.890739766875085No Hit
CAGTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1915500.40319301079913167No Hit
GGGATCAGTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAG1289640.2714559302777302No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT1208550.25438732090905275No Hit
CAGGGATCAGTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG1086210.22863601162105185No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA883900.1860518414227891No Hit
ATCAGTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCAC846450.17816900234451843No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC803670.16916425319182363No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG803050.16903374958091502No Hit
TTCAGGGATCAGTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGT785200.16527650852491685No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG710400.14953187933787687No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTC666520.1402955915206668No Hit
GATCAGTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCA616550.12977742146082205No Hit
TTTCAGGGATCAGTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTG587310.12362270277861552No Hit
GAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAG549300.11562198947113707No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG541240.113925442529325No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA519990.10945253651027954No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAACCGG507260.10677300269275258No Hit
GAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGT503080.10589315576759446No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA478030.1006203889074962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAT1416650.014.984671
AGGGATC456900.014.4458752
AGCTTAG256550.014.07063945
GTTGTCG27800.014.00990326
TTCTATA1778300.013.4811922
CAGGGAT504800.013.4717041
GTCACTC365200.013.3703098
GGGAGGT376750.013.1328973
GGTCACT367450.013.0981777
TCTATAG1816850.013.0787393
CACCGAA1791900.013.03906345
GCACCGA1794000.012.99086344
ATAGGGG281700.012.797441538
GGCATAG287200.012.62487435
CTATAGT1905050.012.46024
TATAGTG1923500.012.3642135
ATAGTGT1932400.012.2988646
TAGTGTG1935450.012.214197
AGGTCAC406900.012.1255986
GAGCTTA300150.012.10456544
Produced by FastQC (version 0.11.5)