FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30281438
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG934610.3086412210675068No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT550130.1816723499062363No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA438790.1449039507304772No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG327170.10804308566852076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC106900.026.1821464
GACCGCG108050.025.8600445
ACCGCGT112650.024.7441926
CAGACCG119150.023.7558173
TTCGGCG56350.021.55904244
TCGGCGC58350.020.9354445
TCAGACC142400.020.4933012
GCAACGC79650.017.7691921
CTCAGAC196200.017.0838621
CGTACGC73800.016.9187815
CCGTACG77150.016.3893974
CGGCAGT279650.016.32422840
CCGCGTT171500.016.2137667
TACGCCA78200.016.1105697
TTCGACG11700.015.3828686
GTACGCC82100.015.2357856
AGTTGCA305950.015.19180744
CGCCGCG80750.015.10514128
TCCGTAC84500.015.0976633
CGCGGGG81750.015.05671731
Produced by FastQC (version 0.11.5)