FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19892599
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA230560.11590240169220722No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG221120.11115691820862622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG14050.018.08563826
GGGAGGT122150.014.5010283
TGTCGTT17950.014.40748328
CGTGGCG54250.014.39716151
ATAGGGG79550.014.23472138
GTCACTC118500.014.0751918
GGTCACT119250.013.8902137
AGCTTAG80600.013.87354245
TATAGCT23350.013.8603887
GGAGGTC126000.013.7535024
GGCATAG81400.013.73923735
ACTCTCC121450.013.55245511
ATATAGC23100.013.3292986
CGACCCG58850.013.328396
GTGGCGA58400.013.213582
GAGCTTA90150.012.55632144
GCTAAGT20600.012.55083514
GTCGTTC19900.012.54411329
GAGGTCA139150.012.5348745
TCACTCT137850.012.4592999
Produced by FastQC (version 0.11.5)