FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30621910
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA89700.012.86584545
TACGGTT29150.011.6454635
TTACGGT29350.011.10657934
TAATCGG50850.010.6781084
TATATAC45850.010.6088967
TGTTACG32050.010.31112832
CATGCAT60350.010.2240279
ATAATCG55200.010.2040033
GGTAACG51700.010.01632917
GTAACGA52750.09.85885918
ATCGGTT54850.09.8536426
AATCGGT54650.09.687635
CGGTTGG37550.09.22018637
ATATATA57050.09.1973456
ATAGGGG54150.09.13402838
TTCCATA67750.09.1327762
ATACATG75900.08.8120486
TCGGTTT62500.08.7915537
CCATATA55900.08.7864414
CATATAT60400.08.6907815
Produced by FastQC (version 0.11.5)