FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48982852
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2460060.5022288208126386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC415400.029.5682664
CAGACCG427850.028.8130343
ACCGCGT429700.028.5685546
TCAGACC455000.027.6421052
GACCGCG444850.027.6006685
CCGCGTT474250.025.8836697
CTCAGAC518600.025.4919321
CGCGTTC489850.025.0584478
GCGTTCT523300.023.4953239
CGTTCTC595550.021.04161310
GAACGAT601500.020.31004545
AGAACGA645800.018.9658144
CCTCTCA660700.018.8952419
ATACGGA643600.018.86801533
AATACGG649950.018.73887632
TACGGAG649600.018.6248534
ACTCCCC669550.018.60282925
GTTCTCT680900.018.57588611
CAATACG663000.018.44126331
TCACTCC685700.018.27583523
Produced by FastQC (version 0.11.5)