FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48982852
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA653650.1334446593677314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCTC115100.016.4601829
CGGGACG115500.015.44656417
ATAGGGG119000.015.42708538
CGCTCTC125400.015.28596711
AGCTTAG119150.015.27550545
GCGCTCT126100.015.11224410
GGCATAG122050.014.89409935
ACGTCTC123700.014.53174421
CAGCGCT135050.014.1296828
TCTCGGG130050.014.06447414
CATAGGG132100.013.88019137
GCAGCGC140100.013.701817
TCGGGAC132050.013.66404416
GCTCTCG131450.013.28159412
GACGTCT138300.013.22540720
GGGACGT138650.012.96485118
CTCTCGG143800.012.73536913
GAGCTTA144450.012.61560744
GCATAGG145550.012.5357236
TGGCATA149750.012.27427634
Produced by FastQC (version 0.11.5)