FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40695983
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG963170.23667446489743227No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA921440.22642038158901334No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG691150.16983248690663155No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC517410.12714031259547165No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT453110.11134022736347222No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG430600.10580896890978159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCACT358400.017.890847
GTCACTC360350.017.8063838
AGCTTAG270450.017.64996745
GGGAGGT388900.017.1127993
ATAGGGG284900.016.78378338
GGCATAG285200.016.7524135
GGAGGTC394800.016.5836224
ACTCTCC391450.016.32911911
GAGGTCA402450.016.3187665
GAGCTTA298650.015.95390944
CACTCTC407100.015.78973410
TCACTCT416650.015.6493739
TGGCATA308200.015.56066734
TGGGAGG337150.014.7677492
CATAGGG328650.014.68714537
CTGGGAG382250.014.2907881
AGGGATC169500.014.0699382
GCATAGG352300.013.70161336
TCTCCCC475000.013.57060913
AGGTCAC498200.013.1462866
Produced by FastQC (version 0.11.5)