FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45705414
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG748960.16386680142531912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG253750.016.21403945
GGGAGGT385750.015.0363343
ATAGGGG276800.014.73848438
GGAGGTC385400.014.6939594
GGCATAG285100.014.35036635
GGTCACT393750.014.1194757
GTTGTCG33800.014.11298426
GTCACTC406750.013.7730948
GAGGTCA416250.013.7671925
GAGCTTA302250.013.62047344
TGGCATA305850.013.3915334
ACTCTCC433500.013.02732711
CACTCTC448750.012.5043810
TGGGAGG356400.012.20268252
CATAGGG340950.012.170567537
TCACTCT477300.012.0157799
GCATAGG356700.011.65241136
TAGGGGA492400.011.30102139
TGTCGTT43050.011.13345428
CTGGGAG430450.011.1279111
Produced by FastQC (version 0.11.5)