FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45933466
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1716840.37376669986105554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG345650.026.3856125
AGACCGC348450.026.3154034
CAGACCG352300.026.0663743
ACCGCGT354600.025.8651226
TCAGACC391850.023.9754122
CTCAGAC436550.022.7635351
CCGCGTT404850.022.6580567
CGCGTTC423700.021.6500198
TTCGACG19350.020.583136
GCGTTCT466400.019.7056669
CGTTCTC514800.018.19825710
GTTCTCT619700.015.25933211
GAACGAT599300.014.85479445
TCGACGA28700.014.8168527
CCTCTCA632450.014.5105619
CGGTATA35300.014.46794520
CTCTCCC647900.014.3099814
GTTCGAC28800.014.2983435
CACTCCC663250.013.95996224
TCTCCCT670400.013.92031715
Produced by FastQC (version 0.11.5)