FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45933466
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA765480.16664973638174835No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG559310.12176525063447204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG48750.018.73906326
ATATAGC62550.016.7240056
TGTCGTT56400.016.39694428
GTCGTTC58050.015.73592829
TATAGCT72000.014.5915347
GCTAAGT66400.014.39871314
GGGAGGT293050.014.2638743
GGAGGTC291500.014.1544474
TTGTCGT66400.014.0290827
TCGTTCC65150.013.98726430
GTCACTC288950.013.663628
GAGGTCA304450.013.5592525
ATAGGGG212400.013.5388238
AGCTTAG211850.013.53017345
GGTCACT292850.013.5048627
GGCATAG214750.013.37008835
CAATATA84400.013.061284
AATATAG100950.012.6132125
TGGCATA236950.012.27854134
ACTCTCC322650.012.25704511
Produced by FastQC (version 0.11.5)