FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47668152
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT807400.16937933738232605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG38100.026.519146
AGACCGC168750.023.4178264
GACCGCG169100.023.3160275
ACCGCGT175250.022.547826
CAGACCG185250.021.5385463
TCGACGA47950.021.4897827
CGGTATA64850.021.05881120
CGACGAG48200.021.0050338
GTTCGAC52750.019.1125935
CCGCGTT207000.019.0857547
TCAGACC225550.018.1322152
CGCGTTC223250.017.6563348
AACGACG26900.017.3134916
TTCGGCG62700.016.21927844
CCCGTAT27650.016.1179641
ATGACGG78650.016.10557416
CTCAGAC293550.015.841211
ACGAGGA89000.015.11867510
GTATAGA99950.015.104122
TCGGCGC67700.015.021399545
Produced by FastQC (version 0.11.5)