FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32970232
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA907390.2752149272107033No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG640500.19426614893095082No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT474990.14406632018846577No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC454940.1379850769627584No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG412650.1251583549669896No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA401970.12191906929863278No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG367930.11159460449049918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGTC247250.015.84997754
GGGAGGT264800.015.6242193
CGTGGCG98900.015.4997331
AGGTCAC257050.015.087876
AGGGATC124050.015.0791782
GAGGTCA264050.014.85858255
GTGGCGA105800.014.4676062
GTCACTC268350.014.2409088
ACTCTCC279400.013.645493511
TCACTCT283500.013.6069049
TGGCGAC113200.013.5615323
CACTCTC278350.013.51097910
TTCTATA418900.013.0362862
TTTCTAT392900.012.9020821
GGCGACC119650.012.7738914
CGACCCG123650.012.5425366
CAGGGAT162700.012.4655271
GCGACCC125750.012.4048475
GGTCACT308400.012.3845697
TCTATAG439550.012.2086813
Produced by FastQC (version 0.11.5)