FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10209095
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440650.4316249383515385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGT8550.015.7923781
CCCGTTA5050.011.58560853
GTCGTAT7500.011.09796229
GGGGGAG40600.010.7532091
CGCCCAT8500.010.5901821
CCGTTAT5750.010.5676784
CCCCCTT36400.010.5101751
AGTCGTA7950.010.18695928
CCCCGTT10450.010.1208342
CCCTCGT9900.010.001841
CGCCGAT2251.6428085E-410.0018391
GCCCGTT8600.09.9436891
CCGTTAA2504.344453E-59.9023064
ATTAGAC17450.09.8016137
ATTACGC2352.5528262E-49.57346241
GTCGGTT6550.09.2765161
CGTACGC2701.00922E-49.16812710
CGGGGGA11350.09.120621
GGATTAG21050.09.0874935
GTCCCGT7000.09.0016561
Produced by FastQC (version 0.11.5)