FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42024550
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG92150.010.27719738
GTTGTCG26600.09.72606626
AGCTTAG96150.09.49882445
ATATAGC35750.09.1281226
GGCATAG100700.09.09219835
CATAGGG110150.08.29136137
GGGAGGT160000.08.2321073
GTCACTC152300.07.8757798
GCATAGG116250.07.856343336
GAGCTTA121600.07.714273544
GGAGGTC165450.07.60722734
ACTCTCC165450.07.507049611
TGTCGTT33400.07.409112528
AACTCTA94250.07.39900745
TATAGCT43550.07.38976577
GGTCACT161350.07.32273157
AGGTCAC176150.07.21863846
ATTCGAC61600.07.09085665
TGGCATA133350.07.05175234
GGCGTAG40950.07.03169435
Produced by FastQC (version 0.11.5)