FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37220679
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT608000.1633500560266512No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG572090.15370219334257712No Hit
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA504200.13546233264578544No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA459980.12358184008411023No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTT431960.11605376677840831No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG390770.1049873378183133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG103750.017.1848645
GTTGTCG23950.017.03885326
TGTCGTT26400.015.63031828
ATAGGGG115200.015.39237138
ATATAGC30900.014.6019096
GGCATAG125050.014.32064235
CATAGGG126600.014.13123837
GTCGTTC30100.013.70928129
GTCACTC175900.013.4410178
GCATAGG134100.013.34089936
GCTAAGT31000.013.26116314
AGGGATC177550.013.2440242
TTGTCGT32700.013.16925227
ACCCGGC39600.012.9802875
CAGGGAT201150.012.4832581
TGGCATA145650.012.43179634
ACTCTCC193500.012.31648811
GGTCACT192800.012.2733937
GGGAGGT204950.012.2304163
GTTCTAT239450.012.1921511
Produced by FastQC (version 0.11.5)