FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32272645
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG962780.29832695770675133No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA607940.1883762548746779No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG361300.11195239807583171No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT344150.10663830002158176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC79500.022.6688424
TTCGACG15200.020.721616
CAGACCG89150.020.5946123
GACCGCG95100.018.8308645
ACCGCGT97900.018.2004246
CCGCGTT173050.017.7152541
CGGCAGT305200.016.49815840
TTCGGCG57450.016.3685644
TCAGACC118350.016.0837352
CGGTATA31150.015.96363620
TCGACGA21000.015.7482777
AGTTGCA325950.015.59845944
TCGGCGC61700.015.45981345
GACGGCA323000.015.4360438
TGACGGC326300.015.28007137
CGCGTTC200200.015.23984052
CAGTTGC336750.015.06521443
ACGGCAG334150.015.01509239
CGTACGC65950.014.8733715
CCGTACG65750.014.7141994
Produced by FastQC (version 0.11.5)