FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32272645
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA585060.18128665933641325No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG561630.17402664082847874No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG548500.16995817975254277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG153000.017.47949845
ATAGGGG170650.015.99610638
GGCATAG172500.015.67421835
GGGAGGT253900.015.0533973
GAGCTTA181300.014.92501244
GGAGGTC254200.014.6910874
TGGCATA186650.014.62916334
CATAGGG194050.014.00658437
GTCACTC265000.013.969018
GGTCACT260800.013.9689157
ACTCTCC264450.013.92270911
GCATAGG206750.013.24233936
CACTCTC285200.012.85395310
AGGTCAC295900.012.7296876
TAGGGGA289050.012.44983539
TCACTCT304950.012.2942329
TCTCCCC301900.012.24743113
TGGGAGG241650.012.1733062
GAGGTCA312350.012.1358495
AGGGATC122800.012.05999852
Produced by FastQC (version 0.11.5)