FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40413346
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1215610.30079419803547075No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG445200.11016162828982286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC262350.024.9738544
CAGACCG271050.024.2303453
GACCGCG272850.023.8809015
ACCGCGT277450.023.52556
TCAGACC311050.021.6358872
TTCGACG18800.021.5347966
CTCAGAC365000.019.8998621
CCGCGTT334650.019.5044067
CGCGTTC356100.018.3105938
GTTCCTA77350.017.53408815
GCGTTCT390350.016.6694169
CGGTATA30400.016.13145420
CCTACTC87750.015.61007318
TCGACGA27100.015.1052627
CGACGAG27300.014.5826618
CCCGTAT23450.014.4891691
CGTTCTC461350.014.42582310
TTCGGCG71400.014.27657144
CTACTCT96550.014.16505619
GTCCGTT33600.013.9294341
Produced by FastQC (version 0.11.5)