FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29867882
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT726500.24323787003042263No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC338610.11336927071025658No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC310800.10405826566476993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC135900.025.156954
TTCGACG19100.025.0819846
GACCGCG140600.024.2360125
ACCGCGT143150.023.8818456
CAGACCG144400.023.8785973
CGGTATA30450.023.19356320
CCGCGTT154000.022.1995227
CGCGTTC160700.021.2602568
TCAGACC169500.020.7807042
TTCGGCG81700.020.13618544
TCGACGA26450.019.3028287
TCGGCGC89750.018.20145845
GCGTTCT191500.017.9465899
CGACGAG27250.017.828468
CTCAGAC224050.017.7278841
GTTCGAC29100.016.540735
AACGACG15800.016.51410716
GTATAGA47950.015.61998922
AACGCTA93950.014.84304223
CCGTACG113350.014.7236754
Produced by FastQC (version 0.11.5)