FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29867882
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGC441080.14767702644599975No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG435530.14581884313055743No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCT378880.1268519810008624No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG342000.11450426916779703No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG299980.10043564521916887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTC140550.013.7529158
AGCTTAG115200.013.299170545
TGCCCGT164250.013.12167744
GGTCACT147250.012.960637
TCAGACG77400.012.590472
GGCATAG121150.012.571650535
ATAGGGG124750.012.40729438
AGGTCAC162150.012.3804486
ACTCTCC159500.012.23158911
AGGGATC102050.012.1736582
GGGAGGT173400.011.9537883
TCACTCT168050.011.6497569
CATAGGG132850.011.5831537
ATAGCTC44200.011.50313339
GAGGTCA177950.011.2814475
TGGCATA136400.011.26484934
GAGCTTA139550.011.23652844
ACCCGGC49750.011.2190625
GCCCGTG194900.011.06972245
CACTCTC173550.010.99510410
Produced by FastQC (version 0.11.5)