FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27656163
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG306540.11083967070920142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCTT127350.016.4146732
GCCGGCT117350.016.2063081
CTCGGTT52300.015.1048341
GTCCGTT23000.015.0696571
TTCACGC135350.014.7771487
CCCGTAT23000.014.7760931
GCACTAC42100.014.75171325
AACGCTA129350.014.68160123
ACGCTAC131250.014.48644624
CGCTACC132800.014.35161725
CGGCTTC155150.014.2845353
TCACGCT140750.014.0823598
CTACACG47950.013.79454645
AAAACGC141750.013.49101421
ACGCTCA151550.013.242110
CTCGCGT7700.013.1532261
TCGCGTG4500.013.0009862
CACGCTC156050.012.961169
AAACGCT148700.012.92168622
GAAAACG148050.012.76496420
Produced by FastQC (version 0.11.5)