FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48091685
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA607280.12627546737029488No Hit
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA580480.12070277845328148No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC503500.10469585334762133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG142200.015.58426138
AGCTTAG146400.015.19864345
GGCATAG151650.014.5241235
CATAGGG160100.013.77159837
AGGGATC174100.013.667432
TGGCATA170600.013.16137134
GAGCTTA170250.013.08270944
GTTGTCG26300.012.91727226
GGGAGGT247550.012.6583213
ACCTAGC50000.012.602576
GCATAGG173950.012.55869236
ACTCTCC246200.012.32755911
GGTCACT243950.012.2407937
GTTCTAT241000.012.2369581
GTCACTC248950.012.2288948
TTCTATA504550.011.845982
GGAGGTC262800.011.82864
GCATTCG43000.011.6744443
TCTATAG512000.011.5106413
CAGGGAT228050.011.401741
Produced by FastQC (version 0.11.5)