FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44279057
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTGGGGCTTGTCCT769390.17375934632031573No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA501960.11336284781313206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGC133450.029.0832966
GACCTAG146800.026.8995975
TGACCTA161950.024.5075054
CTCTGAC232950.019.1912981
TCTGACC220450.018.8814032
TAGCAGT229900.017.7039159
GCAGTCA281150.014.50097311
AGCTTAG113200.013.69230745
GGCATAG115350.013.48133335
ATAGGGG119450.013.22395738
CTGACCT330350.013.0359063
CAGTCAA315450.012.92389512
AGTCAAC317050.012.67415213
CTAGCAG330550.012.5174178
CATAGGG127200.012.22427337
GTCACTC176000.011.95278558
CCTAGCA340450.011.9089537
GTGACTC211000.011.825562
GCATAGG132050.011.79277436
GTCAACA347200.011.67059114
Produced by FastQC (version 0.11.5)