FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50772023
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1448340.28526340185420623No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG517870.10199908717444645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC315750.024.2102494
CAGACCG328550.023.4653173
GACCGCG329900.023.049095
ACCGCGT341350.022.3555986
CCGCGTT366100.020.7823737
TCAGACC389000.020.4887332
CGCGTTC374600.020.3289478
CTCAGAC430050.019.6630841
TTCGACG20200.019.629126
GCGTTCT402100.018.9329959
GAACGAT411650.018.2751645
AACGCTA188950.016.93415523
ACGCTAC190350.016.9046124
ATACGGA466400.016.1109533
AATACGG467950.016.10089532
CGCTACC200100.016.0696925
TACGGAG462900.016.01507834
AGAACGA472550.015.9592144
CAATACG484000.015.68771731
CGTTCTC507900.015.50658410
Produced by FastQC (version 0.11.5)