FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37412122
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG642080.17162351817413618No Hit
CTCGGTTTTATTCTTATTGCTCAGGCTTGAGTGCACTGGCCTCTTCTCAGC593740.15870257239084168No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC484670.12954891999977974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTT134400.024.7193831
TCGGTTT152500.021.5288582
CGGTTTT161700.020.2460253
AGTGCAC142850.020.164930
TTATTGC149650.019.75431314
CTTATTG157550.018.50673913
GAGTGCA158450.018.49217629
TGAGTGC157450.018.4809628
GCCGGCT199300.017.8782141
GTGCACT161550.017.80280731
TATTGCT170000.017.4690115
GTCCGTT28100.017.3020651
CCGGCTT224900.017.2597792
GGTTTTA185600.017.2267344
TTGAGTG174300.016.65553927
AACGCTA216300.016.40483323
ACGCTAC218400.016.27813324
TTCACGC228900.016.1782237
CGCTACC219900.016.16759325
GTTTTAT195250.016.0872275
Produced by FastQC (version 0.11.5)