FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11535026
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA130280.11294296172371003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG7000.013.81980226
GGGAGGT60900.013.4594143
GGTCACT59250.013.3306097
GTCACTC59250.013.2534458
ATAGGGG46100.012.68831238
GGAGGTC64950.012.133334
GGCATAG47900.012.11757335
AGCTTAG48950.012.04148445
GCGAACG6750.011.66529519
GAGGTCA70300.011.4972635
ACTCTCC69800.011.34556711
TCACTCT72400.011.3426359
GAGCTTA54450.011.1970344
TGGCATA52150.011.173182534
CATAGGG54500.010.9803637
AGACTCA48000.010.9765474
CACTCTC71750.010.9433910
GCATAGG56750.010.46572936
AGGGATC36200.010.4506362
TCAGACG23250.010.1697052
Produced by FastQC (version 0.11.5)