FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38382177
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA496690.1294064169419051No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG486510.1267541442477325No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG466720.1215981052872535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG147550.016.82691645
GGCATAG164700.015.18830435
GAGCTTA171750.014.5868544
ATAGGGG174150.014.40816338
GTCACTC236100.014.0956728
TGGCATA179400.013.98088534
GGTCACT238900.013.8555317
CATAGGG182950.013.79942637
AGGTCAC253150.013.30647856
ACTCTCC259500.012.93801611
GCATAGG196450.012.89526736
GGGAGGT289350.012.6648413
GAGGTCA271550.012.6496875
GGAGGTC272100.012.5826714
TCACTCT271250.012.4932889
CACTCTC278350.011.91592610
ACCCGGC63900.011.7305515
AGGGATC133050.011.30087952
TAGGGGA297100.011.21050939
GTCCAAG315650.010.80262327
Produced by FastQC (version 0.11.5)