FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40760550
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2115420.5189871088589334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC438200.028.4510234
CAGACCG446100.028.018293
GACCGCG469950.026.5190895
CGCGTTT68300.026.3850738
ACCGCGT490750.025.4900726
CTCAGAC529600.024.9617311
TCAGACC520000.024.5557332
CCGCGTT516450.024.2170527
GAACGAT513100.023.52227245
CGCGTTC469700.022.8094778
GCGTTCT469200.022.7570089
AATACGG569950.021.36694532
ATACGGA571100.021.28436933
AGAACGA571700.021.17138944
TACGGAG576500.021.01470434
CCCAATA588150.020.7721629
CAATACG587150.020.75302531
CGTTCTC530350.020.38749910
CACTCCC615650.020.1561324
ACGGAGA605850.020.07087935
Produced by FastQC (version 0.11.5)