FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40760550
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA468650.11497636808139242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC68200.012.93267255
ATAGCTC71050.011.43159439
TCACCCG81550.011.36743453
AGCTTAG104050.011.05176145
GTCACTC156350.010.7496248
GGTCACT154650.010.6351047
TAGCTCT78400.010.61799240
ATAGGGG110550.010.399857538
GGCATAG109900.010.38043435
TAGGCTA64200.010.09331710
ACGGACA88650.09.94878318
AGGTCAC173350.09.9420286
ACTCTCC169750.09.68907711
CGACCTA47850.09.6392348
TATCCTA71400.09.6114355
AGGGATC129250.09.5920012
GGGAGGT206450.09.4273683
CACCCGG96000.09.4221594
ATCCTAG83950.09.3267136
CGGACAG94800.09.18458219
Produced by FastQC (version 0.11.5)