FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32305017
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG431680.13362630330762557No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC343220.10624355963038186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG63200.024.9878045
AGACCGC63250.024.9324844
ACCGCGT66150.023.8734596
CAGACCG68600.023.4471473
CCGTACG32550.016.8634684
CGTACGC32550.016.5870195
TCAGACC100400.016.5360372
CGCGTAA6050.016.3739091
CTCGGTT80800.016.2168581
CCGCGTT100250.015.7526927
CTCAGAC132150.015.2649691
TCCGTAC38550.014.1804493
TACGCCA39050.014.1139017
CTACACG76800.013.91494545
TTCGACG13550.013.6139036
CGCGTTC118450.013.3892458
CGGTATA19350.013.13811720
TCGGTTT91600.012.8935522
GTACGCC43800.012.6861826
GCCTACG16150.012.53606510
Produced by FastQC (version 0.11.5)