FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38175496
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG562740.14740869378619206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA192200.016.1705423
TTCACGC201500.015.9819757
ACGCTAC195250.015.94105824
CGCTACC197250.015.74565425
CCGGCTT220600.015.7300142
CCCGTAT26800.015.1950641
AAAACGC206600.015.10829121
TCACGCT216700.014.9540748
GCACTAC69750.014.89641725
GAAAACG210850.014.64354620
GCCGGCT216800.014.4145711
GTCCGTT31250.014.3992121
AAACGCT219750.014.2350322
CCCGGAT81900.014.229991
CTACACG67650.014.13403645
ACGCTCA230700.014.07581410
AGACCGC53700.014.0294044
CGGCTTC267950.013.546233
GTTTTCG227100.013.41363945
CACGCTC246600.013.1956079
Produced by FastQC (version 0.11.5)