FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37333949
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTC194350.013.2268528
GGTCACT195850.013.0797337
GGGAGGT213950.012.9832383
CGTGGCG73000.012.3340161
GGAGGTC221800.012.1279264
ACTCTCC211600.011.97698811
TCACTCT224000.011.6668119
CGTAGGG78100.011.6070337
CACTCTC217400.011.54422210
GGCGTAG78500.011.51923335
GCGTAGG77700.011.49308636
ATAGGGG146400.011.27774138
GAGGTCA239800.011.255125
TCTATAG265200.011.1023373
TTCTATA274250.011.0314552
TTTCTAT278350.010.981861
GGCATAG149300.010.86282135
GTGGCGA85450.010.4845832
TGGCGTA86350.010.47202934
GTTGTCG16500.010.36117526
Produced by FastQC (version 0.11.5)