FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48497809
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA519630.10714504649065693No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG495740.102219050761654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG33650.013.43489626
GGCATAG116550.011.1555735
ATAGGGG120000.011.120584538
CACCGAA441700.010.80460345
AGCTTAG123300.010.68053845
AGGGATC185850.010.6214222
GCACCGA448850.010.53231344
CATAGGG126750.010.43759837
GTTCTAT188350.010.4085471
GCATAGG131800.010.03643336
GCTAAGT47100.010.03359514
GTCACTC171250.010.0129078
GGGAGGT199400.09.8665753
GGTCACT175300.09.69135957
ATATAGC56000.09.6433236
TGTCGTT46400.09.54879928
TTCTATA544600.09.3860992
ACCCGGC60550.09.3685895
TCGTTCC48800.09.30911330
TCTATAG537800.09.27951053
Produced by FastQC (version 0.11.5)