FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35443894
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT752450.2122932655198664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC152200.029.161964
CAGACCG158400.028.1767393
ACCGCGT161300.027.4470086
GACCGCG166650.026.552375
TCAGACC193850.024.0685022
CTCAGAC225300.022.716411
CCGCGTT197650.022.3761547
CGCGTTC217100.020.4128918
GCGTTCT238300.018.6814829
CGTTCTC286900.016.12063410
CCGTACG28750.015.5711084
CGTACGC29450.015.5065435
TACGCCA34100.013.3258757
CTACACG41400.013.205296545
CGCGTAA9400.013.1745651
GTTCTCT362550.013.08573811
CGCGTGT9150.013.0423621
TTCGACG12350.012.9328936
GTCCGTT23400.012.8940811
CTCGGTT53850.012.8785351
Produced by FastQC (version 0.11.5)