FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40270946
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG83900.021.242335
ACCGCGT86750.020.648146
AGACCGC90750.019.7133054
CAGACCG101300.018.082313
CCGCGTT102300.017.3994487
CGCGTTC111900.015.9463598
TCAGACC132500.014.7075882
GTCCGTT35250.014.1713681
CCCGTAT25300.013.8746481
CGTACGC33500.013.0315445
AACGCTA92800.012.87426323
ACGCTAC94100.012.79204624
GTCGGTT32500.012.531791
CCCGCGT12100.012.4596941
GTCCGAT29450.012.4543261
CGCTACC96750.012.30210625
CCGTACG35200.012.0825364
CTCAGAC189000.012.07241251
GCGTTCT151250.011.855539
CTCGCGT9500.011.8430711
Produced by FastQC (version 0.11.5)