FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21644908
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT734700.3394331821599796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC141500.028.9702554
CAGACCG147700.027.9842113
ACCGCGT150400.027.2853376
GACCGCG151750.027.116735
TCAGACC159150.026.1729352
CCGCGTT173200.023.6675227
CGCGTTC178850.022.882118
CTCAGAC207600.021.975171
GCGTTCT198850.020.6485529
TTCGACG9600.020.6235666
CGGTATA14000.019.28539820
CGTTCTC234500.017.76848810
TCGGCGC61200.017.24160845
TTCGGCG62850.017.00394444
AACGCTA58450.016.43727523
ACGCTAC59550.016.2092924
CGTACGC68850.015.9139265
CGCTACC52450.015.7438925
CCGTACG69150.015.64996054
GTTCTCT279950.015.08468111
Produced by FastQC (version 0.11.5)