FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26562102
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG26500.030.3830816
GTTCGAC30150.027.749195
TCGACGA29600.027.5049927
CGGTATA41150.024.65570820
CGACGAG32350.024.541168
AGTTCGA39700.021.527214
GACCGCG46750.021.1192055
ACCGCGT48650.020.6642366
CAGTTCG43050.020.426743
AGACCGC50800.020.0995734
CGAGGAT47200.018.63071311
ATGACGG51400.018.3775416
GTATAGA62800.017.70045322
CAGACCG59000.017.6872983
GACGAGG52850.017.5751789
CCGCGTT57650.017.4382487
TCGTTAC23050.017.18260844
AACGACG19050.017.11883516
CGTTACC23350.016.9594245
CCCGTAT14200.016.9564061
Produced by FastQC (version 0.11.5)