FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30551176
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG310050.10148545509344714No Hit
CAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGG308070.10083736220170378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCTC65700.016.694979
AGCTTAG89000.015.74544245
ACCCGGC50800.015.170245
CGGGACG61950.014.57405817
GGCATAG97600.014.51099635
CAGCGCT76950.014.4297998
ATAGCTC52800.013.75375639
ATAGGGG103450.013.73441438
TCACCCG59900.013.5052753
GCGCTCT82350.013.48715410
GCAGCGC83200.013.4811847
ACGTCTC70450.013.44912921
GAGCTTA105350.013.42889244
CATAGGG107400.013.39759937
TCTCGGG69400.013.10750414
TGGCATA109300.013.01866934
TCGGGAC70850.012.96615716
CGCTCTC86050.012.80206811
TAGCTCT57350.012.58535840
ACGGACA63100.011.98707818
Produced by FastQC (version 0.11.5)