FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36397362
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1455740.3999575573636353No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA868350.23857498243966144No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT492420.13529002459024367No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG450490.12376995893273804No Hit
CCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCC386710.10624671095668967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC106650.022.5038134
GACCGCG116850.020.5971725
CAGACCG120750.020.341963
ACCGCGT128250.018.7826656
CCGCGTT237650.018.7158321
TTCGACG18150.017.3490836
TCAGACC147000.016.9850852
CGCGTTC265100.016.545772
TTCGGCG77400.016.48260944
CGGCAGT430450.016.47975340
AGTTGCA449300.015.874742544
TCGGCGC82600.015.36085745
CAGTTGC473100.015.16827443
CGGTATA26550.014.99483120
ACGGCAG476850.014.84352739
GACGGCA477750.014.75879438
TGACGGC482250.014.65425737
GCGTTCT313200.014.5719473
GTTGCAG492800.014.49403945
CCGTACG90150.014.27193454
Produced by FastQC (version 0.11.5)