FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39870209
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1364890.3423332945157122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG266050.027.3210015
AGACCGC270400.027.0154464
ACCGCGT271250.026.8413166
CAGACCG279900.026.2837523
TCAGACC315800.024.2932012
CCGCGTT300600.024.2051337
CGCGTTC316650.022.9992398
CTCAGAC357200.022.6937771
GCGTTCT352100.020.7283789
GAACGAT365800.019.80778345
AATACGG400800.018.13417632
ATACGGA401200.018.08243233
AGAACGA404850.017.99030544
CGTTCTC422800.017.98570410
TACGGAG398550.017.95991734
CAATACG416100.017.71090531
AAGAACG420250.017.23341643
ACGGAGA422050.016.95983335
CGGAGAG433250.016.32882136
CCCAATA460700.016.27996329
Produced by FastQC (version 0.11.5)