FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39532188
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA832080.21048164599439828No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG722950.18287629311081896No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT668960.16921906776321108No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG666480.16859173087004442No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG656800.16614309331929716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG252300.014.1469245
GTCACTC339650.013.754168
GGTCACT347650.013.4820457
AGGTCAC356700.013.4031926
GTTGTCG26800.013.18866226
ACCCGGG89800.013.129342
GGAGGTC372750.012.9316014
GGGAGGT385050.012.7214773
GAGGTCA382450.012.6340715
GGCATAG286000.012.44826835
TCACTCT382350.012.38975059
CACCCGG98300.012.382771
ATAGGGG289150.012.33438338
ACTCTCC383200.012.27817211
CACTCTC389950.012.05166510
TGGCATA293950.012.02090134
GAGCTTA297350.011.87002544
AACTCTA90350.011.29780345
ATTTCTA307600.010.8198642
TTTCTAT387750.010.68024351
Produced by FastQC (version 0.11.5)