FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43601885
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA578460.1326685761406875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAG248300.012.4145983
TTTCTAT282200.011.5142161
TTCTATA275950.011.4721832
CTATAGT274250.011.3058544
ATAGTGT280850.011.1126286
TATAGTG282500.011.0320215
TAGTGTG286500.010.8374577
TACTAGA299150.010.14530215
TTACTAG299150.010.1228914
GTTACTA303300.09.94013613
ATAGGGG77700.09.81703938
GTGTTAC314050.09.7363711
TGTGTTA318500.09.71315210
ACTAGAG326350.09.27251616
AAGCCGG206500.09.12214345
AGCTTAG85000.09.00225845
GGCATAG84350.08.90965135
TGTTACT353100.08.81245312
CGTCGAT24600.08.78028312
CATAGGG88450.08.70021737
Produced by FastQC (version 0.11.5)