FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40900009
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1172180.28659651395186736No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA411310.10056477004687212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG26800.025.3450456
CGGTATA41600.022.27645320
TCGACGA33000.021.2648667
GTTCGAC34700.018.7970625
AGACCGC51850.018.6960544
CGACGAG35100.018.5187958
CGGCAGT352000.016.66321440
CCGCGTT132200.016.4738221
GACGGCA355000.016.29259138
ACGGCAG360900.016.04815539
TGACGGC361700.015.92926937
AGTTGCA369150.015.86089444
TTCGGCG77950.015.73271544
CCGTACG80900.015.1797844
CGTACGC82300.015.0582055
CAGACCG68050.014.9723493
GTATAGA66150.014.96054722
CAGTTGC393150.014.87125843
ATGACGG59650.014.78228916
TCGGCGC82500.014.75450545
Produced by FastQC (version 0.11.5)