FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44897568
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1111120.24747888348874486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC229000.025.735584
GACCGCG229250.025.5504075
ACCGCGT239350.024.6036436
CAGACCG242150.024.449523
TCAGACC287350.021.5511882
CCGCGTT275450.021.3545537
CGCGTTC282800.020.8068318
CTCAGAC350500.018.9645861
GCGTTCT331350.017.7830629
TTCGGCG58650.014.99938844
CGTTCTC413250.014.93934910
CCGTACG61250.014.9160534
CGTACGC62150.014.5913975
TCGGCGC64750.013.55161645
TACGCCA69200.013.4298657
ACTCCCC464800.013.302278525
CCCGTAT31050.013.2620421
GAACGAT452850.013.13630845
CTCGCGT9800.013.0878911
CACTCCC473900.013.05638324
Produced by FastQC (version 0.11.5)